Sequence variations of ABCB1, SLC6A2, SLC6A3, SLC6A4, CREB1, CRHR1 and NTRK2: association with major depression and antidepressant response in Mexican

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Original Article

Molecular Psychiatry advance online publication 20 October 2009; doi: 10.1038/mp.2009.92

C Dong¹, M-L Wong² and J Licinio²

1. Department of Psychiatry and Behavioral Sciences, Center for Pharmacogenomics, University of Miami Miller School of Medicine, Miami, FL, USA
2. John Curtin School of Medical Research, The Australian National University, Canberra, ACT, Australia

Correspondence: Professor J Licinio, John Curtin School of Medical Research, The Australian National University, GPO Box 334, Canberra, ACT 2601, Australia. E-mail: julio.licinio@anu.edu.au

Received 20 July 2009; Accepted 28 July 2009; Published online 20 October 2009.

Abstract

We studied seven genes that reflect events relevant to antidepressant action at four sequential levels: (1) entry into the brain, (2) binding to monoaminergic transporters, and (3) distal effects at the transcription level, resulting in (4) changes in neurotrophin and neuropeptide receptors. Those genes are ATP-binding cassette subfamily B member 1 (ABCB1), the noradrenaline, dopamine, and serotonin transporters (SLC6A2, SLC6A3 and SLC6A4), cyclic AMP-responsive element binding protein 1 (CREB1), corticotropin-releasing hormone receptor 1 (CRHR1) and neurotrophic tyrosine kinase type 2 receptor (NTRK2). Sequence variability for those genes was obtained in exonic and flanking regions. A total of 56 280 000 bp across were sequenced in 536 unrelated Mexican Americans from Los Angeles (264 controls and 272 major depressive disorder (MDD)). We detected in those individuals 419 single nucleotide polymorphisms (SNPs); the nucleotide diversity was 0.000540.0001. Of those, a total of 204 novel SNPs were identified, corresponding to 49% of all previously reported SNPs in those genes: 72 were in untranslated regions, 19 were in coding sequences of which 7 were non-synonymous, 86 were intronic and 27 were in upstream/downstream regions. Several SNPs or haplotypes in ABCB1, SLC6A2, SLC6A3, SLC6A4, CREB1 and NTRK2 were associated with MDD, and in ABCB1, SLC6A2 and NTRK2 with antidepressant response. After controlling for age, gender and baseline 21-item Hamilton Depression Rating Scale (HAM-D21) score, as well as correcting for multiple testing, the relative reduction of HAM-D21 score remained significantly associated with two NTRK2-coding SNPs (rs2289657 and rs56142442) and the haplotype CAG at rs2289658 (splice site), rs2289657 and rs2289656. Further studies in larger independent samples will be needed to confirm these associations. Our data indicate that extensive assessment of sequence variability may contribute to increase understanding of disease susceptibility and drug response. Moreover, these results highlight the importance of direct re-sequencing of key candidate genes in ethnic minority groups in order to discover novel genetic variants that cannot be simply inferred from existing databases.

Urocortin-1 and -2 double-deficient mice show robust anxiolytic phenotype and modified serotonergic activity in anxiety circuits

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Original Article

Molecular Psychiatry advance online publication 3 November 2009; doi: 10.1038/mp.2009.115

A Neufeld-Cohen¹, A K Evans², D Getselter¹, A Spyroglou³, A Hill³, S Gil¹, M Tsoory¹, F Beuschlein³, C A Lowry², W Vale⁴ and A Chen¹

1. Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel
2. Department of Integrative Physiology, University of Colorado, Boulder, CO, USA
3. Medical Clinic, University Hospital Innenstadt, Ludwig Maximilians University, Munich, Germany
4. Clayton Foundation Laboratories for Peptide Biology, The Salk Institute for Biological Studies, La Jolla, CA, USA

Correspondence: Dr A Chen, Department of Neurobiology, Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel. E-mail: alon.chen@weizmann.ac.il

Received 9 January 2009; Revised 21 September 2009; Accepted 23 September 2009; Published online 3 November 2009.

Abstract

The urocortin (Ucn) family of neuropeptides is suggested to be involved in homeostatic coping mechanisms of the central stress response through the activation of corticotropin-releasing factor receptor type 2 (CRFR2). The neuropeptides, Ucn1 and Ucn2, serve as endogenous ligands for the CRFR2, which is highly expressed by the dorsal raphe serotonergic neurons and is suggested to be involved in regulating major component of the central stress response. Here, we describe genetically modified mice in which both Ucn1 and Ucn2 are developmentally deleted. The double knockout mice showed a robust anxiolytic phenotype and altered hypothalamic–pituitary–adrenal axis activity compared with wild-type mice. The significant reduction in anxiety-like behavior observed in these mice was further enhanced after exposure to acute stress, and was correlated with the levels of serotonin and 5-hydroxyindoleacetic acid measured in brain regions associated with anxiety circuits. Thus, we propose that the Ucn/CRFR2 serotonergic system has an important role in regulating homeostatic equilibrium under challenge conditions.

A new mouse model of pediatric autoimmune neuropsychiatric disorders

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Original Article

Molecular Psychiatry advance online publication 11 August 2009; doi: 10.1038/mp.2009.77

Passive transfer of streptococcus-induced antibodies reproduces behavioral disturbances in a mouse model of pediatric autoimmune neuropsychiatric disorders associated with streptococcal infection

K Yaddanapudi 1,2, M Hornig 1,2, R Serge 1, J De Miranda 1, A Baghban 1, G Villar 1 and W I Lipkin 1

1 Center for Infection and Immunity and Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, USA

Correspondence: Dr M Hornig, Center for Infection and Immunity, Mailman School of Public Health,Columbia University, 722 W 168th Street, New York, NY 10032, USA. E-mail: mh2092@columbia.edu

2 These authors contributed equally to this work.

Received 25 February 2009; Revised 11 June 2009; Accepted 15 June 2009; Published online 11 August 2009.

Abstract

Streptococcal infections can induce obsessive-compulsive and tic disorders. In children, this syndrome, frequently associated with disturbances in attention, learning and mood, has been designated pediatric autoimmune neuropsychiatric disorders associated with streptococcal infection (PANDAS). Autoantibodies recognizing central nervous system (CNS) epitopes are found in sera of most PANDAS subjects, but may not be unique to this neuropsychiatric subset. In support of a humoral immune mechanism, clinical improvement often follows plasmapheresis or intravenous immunoglobulin. We recently described a PANDAS mouse model wherein repetitive behaviors correlate with peripheral anti-CNS antibodies and immune deposits in brain following streptococcal immunization. These antibodies are directed against group A beta-hemolytic streptococcus matrix (M) protein and cross-react with molecular targets complement C4 protein and alpha-2-macroglobulin in brain. Here we show additional deficits in motor coordination, learning/memory and social interaction in PANDAS mice, replicating more complex aspects of human disease. Furthermore, we demonstrate for the first time that humoral immunity is necessary and sufficient to induce the syndrome through experiments wherein naive mice are transfused with immunoglobulin G (IgG) from PANDAS mice. Depletion of IgG from donor sera abrogates behavior changes. These functional disturbances link to the autoimmunity-related IgG1 subclass but are not attributable to differences in cytokine profiles. The mode of disrupting blood–brain barrier integrity differentially affects the ultimate CNS distribution of these antibodies and is shown to be an additional important determinant of neuropsychiatric outcomes. This work provides insights into PANDAS pathogenesis and may lead to new strategies for identification and treatment of children at risk for autoimmune brain disorders.

Brains of psychopaths are different

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Original Article

Molecular Psychiatry advance online publication 9 June 2009; doi: 10.1038/mp.2009.40
Altered connections on the road to psychopathy

M C Craig1,2, M Catani1,2, Q Deeley1, R Latham1, E Daly1, R Kanaan3, M Picchioni3, P K McGuire3, T Fahy4 and D G M Murphy1

1. Section of Brain Maturation, Institute of Psychiatry, De Crespigny Park, London, UK
2. Natbrainlab, Institute of Psychiatry, De Crespigny Park, London, UK
3. Section of Neuroimaging, Institute of Psychiatry, De Crespigny Park, London, UK
4. Department of Forensic Mental Health Science, Institute of Psychiatry, De Crespigny Park, London, UK

Correspondence: Dr MC Craig, Psychological Medicine, Institute of Psychiatry, PO50, 16 De Crespigny Park, Denmark Hill, London SE5 8AF, UK. E-mail: m.craig@iop.kcl.ac.uk

Received 7 August 2008; Revised 25 February 2009; Accepted 13 April 2009; Published online 9 June 2009.

Abstract

Psychopathy is strongly associated with serious criminal behaviour (for example, rape and murder) and recidivism. However, the biological basis of psychopathy remains poorly understood. Earlier studies suggested that dysfunction of the amygdala and/or orbitofrontal cortex (OFC) may underpin psychopathy. Nobody, however, has ever studied the white matter connections (such as the uncinate fasciculus (UF)) linking these structures in psychopaths. Therefore, we used in vivo diffusion tensor magnetic resonance imaging (DT-MRI) tractography to analyse the microstructural integrity of the UF in psychopaths (defined by a Psychopathy Checklist Revised (PCL-R) score of greater than or equal to25) with convictions that included attempted murder, manslaughter, multiple rape with strangulation and false imprisonment. We report significantly reduced fractional anisotropy (FA) (P<0.003),>post hoc comparison with a psychiatric control group with a past history of drug abuse and institutionalization. Our findings remained significant. Taken together, these results suggest that abnormalities in a specific amygdala–OFC limbic network underpin the neurobiological basis of psychopathy.

BDNF and depression: the plot thickens

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Original Article

Molecular Psychiatry advance online publication 21 July 2009; doi: 10.1038/mp.2009.67

Knockdown of brain-derived neurotrophic factor in specific brain sites precipitates behaviors associated with depression and reduces neurogenesis

MPOpen

D Taliaz, N Stall, D E Dar and A Zangen

Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel

Correspondence: Dr A Zangen, Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel. E-mail: a.zangen@weizmann.ac.il

Received 19 December 2008; Revised 29 April 2009; Accepted 15 June 2009; Published online 21 July 2009.

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Abstract

Depression has been associated with reduced expression of brain-derived neurotrophic factor (BDNF) in the hippocampus. In addition, animal studies suggest an association between reduced hippocampal neurogenesis and depressive-like behavior. These associations were predominantly established based on responses to antidepressant drugs and alterations in BDNF levels and neurogenesis in depressive patients or animal models for depressive behavior. Nevertheless, there is no direct evidence that the actual reduction of the BDNF protein in specific brain sites can induce depressive-like behaviors or affect neurogenesis in vivo. Using BDNF knockdown by RNA interference and lentiviral vectors injected into specific subregions of the hippocampus we show that a reduction in BDNF expression in the dentate gyrus, but not the CA3, reduces neurogenesis and affects behaviors associated with depression. Moreover, we show that BDNF has a critical function in neuronal differentiation, but not proliferation in vivo. Finally, we found that a specific BDNF knockdown in the ventral subiculum induces anhedonic-like behavior. These findings provide substantial support for the neurotrophic hypothesis of depression and specify anatomical and neurochemical targets for potential antidepressant interventions. Moreover, the specific effect of BDNF reduction on neuronal differentiation has broader implications for the study of neurodevelopment and neurodegenerative diseases.

Biomarkers for Post-partum Depression

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Original Article

Molecular Psychiatry advance online publication 7 July 2009; doi: 10.1038/mp.2009.65

Blood mononuclear cell gene expression signature of postpartum depression

R H Segman1, T Goltser-Dubner1,5, I Weiner2,5, L Canetti1, E Galili-Weisstub1, A Milwidsky3,4, V Pablov1, N Friedman2 and D Hochner-Celnikier3,4

1. Department of Psychiatry, Hadassah-Hebrew University Medical Center, Jerusalem, Israel

2. School of Computer Science and Engineering, Hebrew University, Jerusalem, Israel

3. Child & Adolescent Psychiatry Unit, Department of Psychiatry, Hadassah-Hebrew University Medical Center, Jerusalem, Israel

4. Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel

Correspondence: Dr RH Segman, Department of Psychiatry, Hadassah-Hebrew University Medical Center, POB 24035 Mount Scopus, Jerusalem 91240, Israel. E-mail: ronense@ekmd.huji.ac.il

5These two authors contributed equally to this work.

Received 25 November 2008; Revised 30 April 2009; Accepted 9 June 2009; Published online 7 July 2009.

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Abstract

In sorrow thou shalt bring forth children (Genesis 3:16) seems as relevant today, with one of seven mothers afflicted by a depressive episode, constituting the most common medical complication after delivery. Why mothers are variably affected by mood symptoms postpartum remains unclear, and the pathogenesis and early molecular indicators of this divergent outcome have not been described. We applied a case–control design comparing differential global gene expression profiles in blood mononuclear cells sampled shortly after delivery at the time of inception of postpartum depression (PD). Nine antidepressant naive mothers showing high depressive scores and developing a persisting major depressive episode with postpartum onset were compared with 10 mothers showing low depressive scores and no depressive symptoms on prospective follow-up. A distinctive gene expression signature was observed after delivery among mothers with an emergent PD, with a significant overabundance of transcripts showing a high-fold differential expression between groups, and correlating with depressive symptom severity among all mothers. Early expression signatures correctly classified the majority of PD patients and controls. Those developing persisting PD exhibit a relative downregulation of transcription after delivery, with differential immune activation, and decreased transcriptional engagement in cell proliferation, and DNA replication and repair processes. Our data provide initial evidence indicating that blood cells sampled shortly after delivery may harbor valuable prognostic information for identifying the onset of persisting PD. Some of the informative transcripts and pathways may be implicated in the differential vulnerability that underlies depression pathogenesis.

Inherited aspects of cognition become more evident with age

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Original Article

Molecular Psychiatry advance online publication 2 June 2009; doi: 10.1038/mp.2009.55

The heritability of general cognitive ability increases linearly from childhood to young adulthood

C M A Haworth¹, M J Wright², M Luciano², N G Martin², E J C de Geus³, C E M van Beijsterveldt³, M Bartels³, D Posthuma^3,4,5, D I Boomsma³, O S P Davis¹, Y Kovas¹, R P Corley⁶, J C DeFries⁶, J K Hewitt⁶, R K Olson⁶, S-A Rhea⁶, S J Wadsworth⁶, W G Iacono⁷, M McGue⁷, L A Thompson⁸, S A Hart⁹, S A Petrill⁹, D Lubinski¹⁰ and R Plomin¹

1. King's College London, Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, London, UK
2. Queensland Institute of Medical Research, Brisbane, QLD, Australia
3. Faculty of Psychology and Education, Department of Biological Psychology, VU University, Amsterdam, The Netherlands
4. Section of Medical Genomics, VU Medical Centre, Amsterdam, The Netherlands
5. Section of Functional Genomics, Faculty of Earth and Life Science, VU University, Amsterdam, The Netherlands
6. Institute for Behavioral Genetics, University of Colorado at Boulder, Boulder, CO, USA
7. Department of Psychology, University of Minnesota, Minneapolis, MN, USA
8. Department of Psychology, Case Western Reserve University, Cleveland, OH, USA
9. Human Development and Family Science, Ohio State University, Columbus, OH, USA
10. Department of Psychology and Human Development, Vanderbilt University, Nashville, TN, USA

Correspondence: Professor R Plomin, King's College London, Social Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, De Crespigny Park, London SE5 8AF, UK. E-mail: Robert.Plomin@iop.kcl.ac.uk

Received 6 February 2009; Revised 27 April 2009; Accepted 4 May 2009; Published online 2 June 2009.

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Abstract

Although common sense suggests that environmental influences increasingly account for individual differences in behavior as experiences accumulate during the course of life, this hypothesis has not previously been tested, in part because of the large sample sizes needed for an adequately powered analysis. Here we show for general cognitive ability that, to the contrary, genetic influence increases with age. The heritability of general cognitive ability increases significantly and linearly from 41% in childhood (9 years) to 55% in adolescence (12 years) and to 66% in young adulthood (17 years) in a sample of 11 000 pairs of twins from four countries, a larger sample than all previous studies combined. In addition to its far-reaching implications for neuroscience and molecular genetics, this finding suggests new ways of thinking about the interface between nature and nurture during the school years. Why, despite life's 'slings and arrows of outrageous fortune', do genetically driven differences increasingly account for differences in general cognitive ability? We suggest that the answer lies with genotype–environment correlation: as children grow up, they increasingly select, modify and even create their own experiences in part based on their genetic propensities.

Transgenic primates as research tools: now a reality

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Article

Nature 459, 523-527 (28 May 2009) | doi:10.1038/nature08090; Received 27 September 2008; Accepted 30 April 2009

Generation of transgenic non-human primates with germline transmission

Erika Sasaki¹, Hiroshi Suemizu¹, Akiko Shimada¹, Kisaburo Hanazawa², Ryo Oiwa¹, Michiko Kamioka¹, Ikuo Tomioka^1,3, Yusuke Sotomaru⁵, Reiko Hirakawa^1,3, Tomoo Eto¹, Seiji Shiozawa^1,4, Takuji Maeda^1,4, Mamoru Ito¹, Ryoji Ito¹, Chika Kito¹, Chie Yagihashi¹, Kenji Kawai¹, Hiroyuki Miyoshi⁶, Yoshikuni Tanioka¹, Norikazu Tamaoki¹, Sonoko Habu⁷, Hideyuki Okano⁴ & Tatsuji Nomura¹

1. Central Institute for Experimental Animals, 1430 Nogawa, Miyamae-ku, Kawasaki, Kanagawa 216-0001, Japan
2. Department of Urology, Juntendo University Nerima Hospital 3-1-10 Takanodai, Nerima-ku, Tokyo 177-8521, Japan
3. Center for Integrated Medical Research,
4. Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan
5. Natural Science Centre for Basic Research and Development, Hiroshima University 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan
6. Subteam for Manipulation of Cell Fate, RIKEN BioResource Centre, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
7. Department of Immunology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan

Correspondence to: Erika Sasaki¹Hideyuki Okano⁴ Correspondence and requests for materials should be addressed to E.S. (Email: esasaki@ciea.or.jp) or H.O. (Email: hidokano@sc.itc.keio.ac.jp).

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Abstract

The common marmoset (Callithrix jacchus) is increasingly attractive for use as a non-human primate animal model in biomedical research. It has a relatively high reproduction rate for a primate, making it potentially suitable for transgenic modification. Although several attempts have been made to produce non-human transgenic primates, transgene expression in the somatic tissues of live infants has not been demonstrated by objective analyses such as polymerase chain reaction with reverse transcription or western blots. Here we show that the injection of a self-inactivating lentiviral vector in sucrose solution into marmoset embryos results in transgenic common marmosets that expressed the transgene in several organs. Notably, we achieved germline transmission of the transgene, and the transgenic offspring developed normally. The successful creation of transgenic marmosets provides a new animal model for human disease that has the great advantage of a close genetic relationship with humans. This model will be valuable to many fields of biomedical research.

Meta-analysis of the dose-response relationship of SSRI in obsessive-compulsive disorder

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Original Article

Molecular Psychiatry advance online publication 26 May 2009; doi: 10.1038/mp.2009.50

Meta-analysis of the dose-response relationship of SSRI in obsessive-compulsive disorder

M H Bloch¹, J McGuire¹, A Landeros-Weisenberger¹, J F Leckman¹ and C Pittenger²

1. ¹Yale Child Study Center, Yale University School of Medicine, New Haven, CT, USA
2. ²Department of Psychiatry, Yale University School of Medicine, New Haven, CT, USA

Correspondence: Dr MH Bloch, Child Study Center, Yale University School of Medicine, PO Box 20709, New Haven, CT 06520, USA. E-mail: Michael.bloch@yale.edu

Received 22 January 2009; Accepted 13 April 2009; Published online 26 May 2009.

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Abstract

We sought to determine differences in efficacy and tolerability between different doses of selective serotonin reuptake inhibitors in the treatment of obsessive-compulsive disorder (OCD) using meta-analysis. We identified 9 studies involving 2268 subjects that were randomized, double-blind placebo-controlled clinical trials that compared multiple, fixed-doses of selective serotonin reuptake inhibitors (SSRIs) to each other and to placebo in the treatment of adults with OCD. Change in Y-BOCS score, proportion of treatment responders, and dropouts (all-cause and due to side-effects) were determined for each included study. Weighted mean difference was used to examine mean change in Y-BOCS score. Pooled absolute risk difference was used to examine dichotomous outcomes. Meta-analysis was performed using a fixed effects model in RevMan 4.2.8. We found that compared with either low or medium doses, higher doses of SSRIs were associated with improved treatment efficacy, using either Y-BOCS score or proportion of treatment responders as an outcome. Dose of SSRIs was not associated with the number of all-cause dropouts. Higher doses of SSRIs were associated with significantly higher proportion of dropouts due to side-effects. These results suggests that higher doses of SSRIs are associated with greater efficacy in the treatment of OCD. This SSRI efficacy pattern stands in contrast to other psychiatric disorders like Major Depressive Disorder. This greater treatment efficacy is somewhat counterbalanced by the greater side-effect burden with higher doses of SSRIs. At present, there are insufficient data to generalize these findings to children or adolescents with OCD.

Chromosome 8p: A Review

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Feature Review

Molecular Psychiatry (2009) 14, 563–589; doi:10.1038/mp.2009.2; published online 10 February 2009

Chromosome 8p as a potential hub for developmental neuropsychiatric disorders: implications for schizophrenia, autism and cancer

R Tabarés-Seisdedos¹ and J L R Rubenstein²

1. Teaching Unit of Psychiatry and Psychological Medicine, Department of Medicine, CIBER-SAM, University of Valencia, Valencia, Spain
2. Nina Ireland Laboratory of Developmental Neurobiology, Department of Psychiatry, University of California, San Francisco, CA, USA. E-mail: Rafael.Tabares@uv.es

Correspondence: Professor Dr R Tabarés-Seisdedos, Teaching Unit of Psychiatry and Psychological Medicine, Department of Medicine, University of Valencia, CIBER-SAM, Blasco-Ibáñez 17, 46010 Valencia, Spain; Professor Dr John LR Rubenstein, Nina Ireland Laboratory of Developmental Neurobiology, Department of Psychiatry, University of California, San Francisco, CA 94143, USA. E-mail: John.Rubenstein@ucsf.edu

Received 1 August 2008; Revised 19 December 2008; Accepted 7 January 2009; Published online 10 February 2009.

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Abstract

Defects in genetic and developmental processes are thought to contribute susceptibility to autism and schizophrenia. Presumably, owing to etiological complexity identifying susceptibility genes and abnormalities in the development has been difficult. However, the importance of genes within chromosomal 8p region for neuropsychiatric disorders and cancer is well established. There are 484 annotated genes located on 8p; many are most likely oncogenes and tumor-suppressor genes. Molecular genetics and developmental studies have identified 21 genes in this region (ADRA1A, ARHGEF10, CHRNA2, CHRNA6, CHRNB3, DKK4, DPYSL2, EGR3, FGF17, FGF20, FGFR1, FZD3, LDL, NAT2, NEF3, NRG1, PCM1, PLAT, PPP3CC, SFRP1 and VMAT1/SLC18A1) that are most likely to contribute to neuropsychiatric disorders (schizophrenia, autism, bipolar disorder and depression), neurodegenerative disorders (Parkinson's and Alzheimer's disease) and cancer. Furthermore, at least seven nonprotein-coding RNAs (microRNAs) are located at 8p. Structural variants on 8p, such as copy number variants, microdeletions or microduplications, might also contribute to autism, schizophrenia and other human diseases including cancer. In this review, we consider the current state of evidence from cytogenetic, linkage, association, gene expression and endophenotyping studies for the role of these 8p genes in neuropsychiatric disease. We also describe how a mutation in an 8p gene (Fgf17) results in a mouse with deficits in specific components of social behavior and a reduction in its dorsomedial prefrontal cortex. We finish by discussing the biological connections of 8p with respect to neuropsychiatric disorders and cancer, despite the shortcomings of this evidence.

The Best on Translational Science

Academic Health Science Centres: a revolution in healthcare?

6 March 2009

King’s Health Partners hosted an international conference on 6 March 2009, discussing ‘Academic Health Science Centres: a revolution in healthcare?’

Presentations followed by question and answer sessions were given from a variety of healthcare and research experts from across the globe, within both public and private sector organisations.

The conference saw over 300 attendees from the UK and further afield, and was opened by Sir Alan Langlands, Principal and Chancellor for the University of Dundee. Other speakers throughout the day included representatives from US healthcare organisations and pharmaceutical companies as well as our own internal specialists in both research and clinical delivery.

The day ended on a positive, with a closing note from Ruth Carnall, Chief Executive for NHS London, whereby she gave her support to all the AHSC applicants, in what will be a positive change for the benefit of patients.

Check this conference on the web and download the presentations. They are really outstanding. Click here.

To see an outstanding Translational Science Center application from the Imperial College of Science, Technology and Medicine, click here.

New findings on the genetics of autism: WGAS points to 5p14.1

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Source: Nature AOP doi:10.1038/nature07999

Abstract:
Common genetic variants on 5p14.1 associate with autism spectrum disorders

Kai Wang et al.

Autism spectrum disorders (ASDs) represent a group of childhood neurodevelopmental and neuropsychiatric disorders characterized by deficits in verbal communication, impairment of social interaction, and restricted and repetitive patterns of interests and behaviour. To identify common genetic risk factors underlying ASDs, here we present the results of genome-wide association studies on a cohort of 780 families (3,101 subjects) with affected children, and a second cohort of 1,204 affected subjects and 6,491 control subjects, all of whom were of European ancestry. Six single nucleotide polymorphisms between cadherin 10 (CDH10) and cadherin 9 (CDH9)—two genes encoding neuronal cell-adhesion molecules—revealed strong association signals, with the most significant SNP being rs4307059 (P53.431028, odd ratio51.19). These signals were replicated in two independent cohorts, with combined P values ranging from 7.431028 to 2.1310210. Our results implicate neuronal cell-adhesion molecules in the pathogenesis of ASDs, and represent, to our knowledge, the first demonstration of genome-wide significant association of common variants with susceptibility to ASDs.

Personal technology: Phoning in data

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Far from being just an accessory, mobile phones are starting to be used to collect data in an increasing number of disciplines. Roberta Kwok looks into their potential.

Pre-publication: the effects of stress on decision-making

From Nature Precedings

hdl:10101/npre.2009.2923.1

For link to paper, click here.

Stress impairs decision-making in rats

Lauren K. Jones¹, Taejib Yoon¹, & Jeansok J. Kim²

Correspondence: (Login to view email address)

1. Department of Psychology, University of Washington
2. Department of Psychology and Program in Neurobiology & Behavior, University of Washington

PDF (232.8 KB)

Document Type:

Manuscript

Date:

Received 05 March 2009 02:48 UTC; Posted 05 March 2009

Subjects:

Neuroscience

Tags:

• foraging behaviour
• decision-making
• stress
• rat
• Amygdala

Abstract:

Stress influences various types of memory, but its effects on other cognitive functions are relatively unknown. We investigated the effects of uncontrollable stress on subsequent decision-making in rats, using a computer vision-based water foraging choice task. Stress impaired the animals’ ability to bias their responses toward the larger reward when transitioning from equal to unequal quantities, and this stress effect was dependent on the amygdala.

From SCIENCE: Fear memory in mice is erased by experimental killing of a subpopulation of lateral amygdala neurons

Science 13 March 2009: Vol. 323. no. 5920, pp. 1492 - 1496 DOI: 10.1126/science.1164139

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For podcast interview with author, click here.

Reports

Selective Erasure of a Fear Memory

Jin-Hee Han,^1,2,3 Steven A. Kushner,^1,4 Adelaide P. Yiu,^1,2 Hwa-Lin (Liz) Hsiang,^1,2 Thorsten Buch,⁵ Ari Waisman,⁶ Bruno Bontempi,⁷ Rachael L. Neve,⁸ Paul W. Frankland,^1,2,3 Sheena A. Josselyn^1,2,3*

¹ Program in Neurosciences and Mental Health, Hospital for Sick Children, 555 University Avenue, Toronto, ON, M5G 1X8, Canada.
² Institute of Medical Sciences, University of Toronto, Toronto, ON, M5G 1X8, Canada.
³ Department of Physiology, University of Toronto, Toronto, ON, M5G 1X8, Canada.
⁴ Department of Psychiatry, Erasmus University Medical Center, 3015 CE Rotterdam, Netherlands.
⁵ Department of Pathology, University of Zurich, CH-8057 Zurich, Switzerland.
⁶ I.Medizinische Klinik und Poliklinik, Johannes Gutenberg-Universität Mainz, 55131 Mainz, Germany.
⁷ Centre de Neurosciences Intégratives et Cognitives, CNRS UMR5228 and University of Bordeaux 1, 33405 Talence, France.
⁸ Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

^* To whom correspondence should be addressed. E-mail: sheena.josselyn@sickkids.ca

Is schizophrenia caused by alterations in gene sets associated with synaptic vesicle recycling, transmitter release and cytoskeletal dynamics?

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Molecular Psychiatry advance online publication 3 March 2009; doi: 10.1038/mp.2009.18

Analysis of gene expression in two large schizophrenia cohorts identifies multiple changes associated with nerve terminal function

P R Maycox¹, F Kelly², A Taylor², S Bates², J Reid², R Logendra³, M R Barnes³, C Larminie³, N Jones², M Lennon⁴, C Davies¹, J J Hagan¹, C A Scorer¹, C Angelinetta⁵, T Akbar⁵, S Hirsch⁵, A M Mortimer^5,6, T R E Barnes⁵ and J de Belleroche⁵

1. Psychiatry CEDD, New Frontiers Science Park, GlaxoSmithKline, Harlow, Essex, UK
2. Genetic and Proteomic Sciences, New Frontiers Science Park, GlaxoSmithKline, Harlow, Essex, UK
3. Computational Biology, New Frontiers Science Park, GlaxoSmithKline, Harlow, Essex, UK
4. Statistical Sciences, New Frontiers Science Park, GlaxoSmithKline, Harlow, Essex, UK
5. Division of Neuroscience and Mental Health, Faculty of Medicine, Imperial College London, Hammersmith Hospital, London, UK
6. Academic Unit of Psychiatry, University of Hull, Hull, UK

Correspondence: Professor J de Belleroche, Neurogenetics Group, Division of Neuroscience and Mental Health, Imperial College London, Hammersmith Hospital Campus, Burlington Danes Building, Du Cane Road, London W12 0NN, UK. E-mail: j.belleroche@imperial.ac.uk

Received 20 October 2008; Revised 27 January 2009; Accepted 28 January 2009; Published online 3 March 2009.

Abstract

Schizophrenia is a severe psychiatric disorder with a world-wide prevalence of 1%. The pathophysiology of the illness is not understood, but is thought to have a strong genetic component with some environmental influences on aetiology. To gain further insight into disease mechanism, we used microarray technology to determine the expression of over 30 000 mRNA transcripts in post-mortem tissue from a brain region associated with the pathophysiology of the disease (Brodmann area 10: anterior prefrontal cortex) in 28 schizophrenic and 23 control patients. We then compared our study (Charing Cross Hospital prospective collection) with that of an independent prefrontal cortex dataset from the Harvard Brain Bank. We report the first direct comparison between two independent studies. A total of 51 gene expression changes have been identified that are common between the schizophrenia cohorts, and 49 show the same direction of disease-associated regulation. In particular, changes were observed in gene sets associated with synaptic vesicle recycling, transmitter release and cytoskeletal dynamics. This strongly suggests multiple, small but synergistic changes in gene expression that affect nerve terminal function.

Is the neurogenesis hypothesis of depression and antidepressant action only applicable to the young?

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Molecular Psychiatry advance online publication 13 January 2009; doi: 10.1038/mp.2008.147

Ageing abolishes the effects of fluoxetine on neurogenesis

S Couillard-Despres¹, C Wuertinger¹, M Kandasamy¹, M Caioni¹, K Stadler¹, R Aigner¹, U Bogdahn¹ and L Aigner^1,2

1. Department of Neurology, University of Regensburg, Regensburg, Germany
2. Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria

Correspondence: Dr S Couillard-Despres, Department of Neurology, University of Regensburg, Franz-Josef-Strauss-Allee 11, Regensburg D-93053, Germany. E-mail: sebastien.couillard-despres@klinik.uni-regensburg.de

Received 9 July 2008; Revised 26 November 2008; Accepted 15 December 2008; Published online 13 January 2009.

Abstract

Depression constitutes a widespread condition observed in elderly patients. Recently, it was found that several drugs employed in therapies against depression stimulate hippocampal neurogenesis in young rodents and nonhuman primates. As the rate of neurogenesis is dramatically reduced during ageing, we examined the influences of ageing on neurogenic actions of antidepressants. We tested the impact of fluoxetine, a broadly used antidepressant, on hippocampal neurogenesis in mice of three different age groups (100, 200 and over 400 days of age). Proliferation and survival rate of newly generated cells, as well as the percentage of cells that acquired a neuronal phenotype were analyzed in the hippocampus of mice that received fluoxetine daily in a chronic manner. Surprisingly, the action of fluoxetine on neurogenesis was decreasing as a function of age and was only significant in young animals. Hence, fluoxetine increased survival and the frequency of neuronal marker expression in newly generated cells of the hippocampus in the young adult group (that is 100 days of age) only. No significant effects on neurogenesis could be detected in fluoxetine-treated adult and elderly mice (200 and over 400 days of age). The data indicate that the action of fluoxetine on neurogenesis is highly dependent on the age of the treated individual. Although the function of neurogenesis in the clinical manifestation of depression is currently a matter of speculation, this study clearly shows that the therapeutic effects of antidepressants in elderly patients are not mediated by neurogenesis modulation.

Adolescent alcohol use: an interaction of parenting and dopamine D2 genotype?

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Molecular Psychiatry advance online publication 24 February 2009; doi: 10.1038/mp.2009.4

Interaction between dopamine D2 receptor genotype and parental rule-setting in adolescent alcohol use: evidence for a gene-parenting interaction

C S van der Zwaluw¹, R C M E Engels¹, A A Vermulst¹, B Franke^2,3, J Buitelaar³, R J Verkes³ and R H J Scholte¹

1. Behavioral Science Institute, Radboud University Nijmegen, Nijmegen, The Netherlands
2. Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
3. Department of Psychiatry, Radboud University Nijmegen Medical Centre, Donders Centre for Neuroscience, Nijmegen, The Netherlands

Correspondence: CS van der Zwaluw, Behavioral Science Institute, Radboud University Nijmegen, PO Box 9104, 6500 HE Nijmegen, The Netherlands. E-mail: C.vanderZwaluw@bsi.ru.nl

Received 22 September 2008; Revised 3 December 2008; Accepted 18 December 2008; Published online 24 February 2009.

Abstract

Association studies investigating the link between the dopamine D2 receptor gene (DRD2) and alcohol (mis)use have shown inconsistent results. This may be due to lack of attention for environmental factors. High levels of parental rule-setting are associated with lower levels of adolescent alcohol use and delay of initiation of drinking. We tested whether DRD2 TaqI A (rs1800497) genotype interacts with alcohol-specific parenting practices in predicting the uptake of regular adolescent alcohol use. Non-regular drinkers were selected from a Dutch, nationwide sample of 428 adolescents (mean age 13.4 years at baseline) and participated in a prospective, community-based study with three annual waves. Parental rule-setting was directly and inversely related to adolescent alcohol use over time. For DRD2 genotype no significant main effect was found. DRD2 genotype interacted with parental rule-setting on adolescent alcohol use over time: adolescents, with parents highly permissive toward alcohol consumption and carrying a genotype with the DRD2 A1 (rs1800497T) allele, used significantly more alcohol over time than adolescents without these characteristics. The DRD2 genotype may pose an increased risk for alcohol use and abuse, depending on the presence of environmental risk factors, such as alcohol-specific parenting.

Prenatal cigarette exposure and genes: a pathway to sociopathy

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Molecular Psychiatry advance online publication 3 March 2009; doi: 10.1038/mp.2009.22

Interaction of prenatal exposure to cigarettes and MAOA genotype in pathways to youth antisocial behavior

L S Wakschlag¹, E O Kistner², D S Pine³, G Biesecker¹, K E Pickett⁴, A D Skol⁵, V Dukic², R J R Blair³, B L Leventhal¹, N J Cox⁵, J L Burns¹, K E Kasza², R J Wright⁶ and E H Cook Jr¹

1. Institute for Juvenile Research, Department of Psychiatry, University of Illinois at Chicago, Chicago, IL, USA
2. Department of Health Studies, University of Chicago, Chicago, IL, USA
3. Laboratory of Affective & Developmental Neurosciences, NIMH Intramural Research Program, Bethesda, MD, USA
4. Department of Health Sciences, University of York, York, England
5. Section of Genetic Medicine, Department of Medicine, University of Chicago, Chicago, IL, USA
6. Channing Laboratory, Harvard University Medical School, Boston, MA, USA

Correspondence: Dr LS Wakschlag, Institute for Juvenile Research, Department of Psychiatry, University of Illinois at Chicago, 1747 W. Roosevelt Road, MC747, Chicago, IL 60608, USA. E-mail: lwakschlag@psych.uic.edu

Received 2 October 2008; Revised 29 January 2009; Accepted 3 February 2009; Published online 3 March 2009.

Abstract

Genetic susceptibility to antisocial behavior may increase fetal sensitivity to prenatal exposure to cigarette smoke. Testing putative gene exposure mechanisms requires precise measurement of exposure and outcomes. We tested whether a functional polymorphism in the gene encoding the enzyme monoamine oxidase A (MAOA) interacts with exposure to predict pathways to adolescent antisocial behavior. We assessed both clinical and information-processing outcomes. One hundred seventy-six adolescents and their mothers participated in a follow-up of a pregnancy cohort with well-characterized exposure. A sex-specific pattern of gene exposure interaction was detected. Exposed boys with the low-activity MAOA 5' uVNTR (untranslated region variable number of tandem repeats) genotype were at increased risk for conduct disorder (CD) symptoms. In contrast, exposed girls with the high-activity MAOA uVNTR genotype were at increased risk for both CD symptoms and hostile attribution bias on a face-processing task. There was no evidence of a gene–environment correlation (rGE). Findings suggest that the MAOA uVNTR genotype, prenatal exposure to cigarettes and sex interact to predict antisocial behavior and related information-processing patterns. Future research to replicate and extend these findings should focus on elucidating how gene exposure interactions may shape behavior through associated changes in brain function.

Angiotensin receptor gene polymorphisms and 2-year change in hyperintense lesion volume in men

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Molecular Psychiatry advance online publication 10 March 2009; doi: 10.1038/mp.2009.26

The preliminary data were presented at the 2008 Annual Meeting of the American Association for Geriatric Psychiatry in Orlando, Florida on 17 March 2008.

W D Taylor^1,2, D C Steffens^1,2, A Ashley-Koch^3,4, M E Payne^1,2, J R MacFall^5,2, C F Potocky^3,4 and K R R Krishnan^1,2,6

1. Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, NC, USA
2. The Neuropsychiatric Imaging Research Laboratory, Duke University Medical Center, Durham, NC, USA
3. Department of Medicine, Duke University Medical Center, Durham, NC, USA
4. The Duke Center for Human Genetics, Duke University Medical Center, Durham, NC, USA
5. Department of Radiology, Duke University Medical Center, Durham, NC, USA
6. The Duke-NUS Graduate Medical School Singapore, Singapore

Correspondence: Dr WD Taylor, Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, DUMC Box 3903, Durham, NC 27710, USA. E-mail: Taylo066@mc.duke.edu

Received 20 October 2008; Revised 9 January 2009; Accepted 11 February 2009; Published online 10 March 2009.

Abstract

This longitudinal study examined the relationship between 2-year change in white matter hyperintense lesion (WML) volume and polymorphisms in genes coding for the angiotensin-II type 1 and type 2 receptors, AGTR1 A1166C and AGTR2 C3123A, respectively. 137 depressed and 94 non-depressed participants aged 60 years were enrolled. Standard clinical evaluations were performed on all participants and blood samples obtained for genotyping. 1.5-T MRI (magnetic resonance imaging) data were obtained at baseline and approximately 2 years later. These scans were processed using a semi-automated segmentation process, which allowed for the calculation of WML volume at each time point. Statistical models were tested for the relationship between change in WML volume and genotype, while also controlling for age, sex, diagnostic strata, baseline WML volume and comorbid cerebrovascular risk factors. In men, AGTR1 1166A allele homozygotes exhibited significantly less change in WML volume than 1166C carriers. We also found that men reporting hypertension (HTN) with the AGTR2 3123C allele exhibit less change in WML volume than hypertensive men with the 3123A allele, or men without HTN. There were no significant relationships between these polymorphisms and change in WML volume in women. No significant gene–gene or gene–depression interactions were observed. Our results parallel earlier observed gender differences of the relationship between other renin–angiotensin system polymorphisms and HTN. Further work is needed to determine whether these observed relationships are secondary to polymorphisms affecting response to antihypertensive medication, and whether antihypertensive medications can slow WML progression and lower the risk of morbidity associated with WMLs.

Irritability as a symptom of major depression

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Molecular Psychiatry advance online publication 10 March 2009; doi: 10.1038/mp.2009.20

The importance of irritability as a symptom of major depressive disorder: results from the National Comorbidity Survey Replication

M Fava¹, I Hwang², A J Rush^3,4, N Sampson², E E Walters² and R C Kessler²

1. Department of Psychiatry, Massachusetts General Hospital, Boston, MA, USA
2. Department of Health Care Policy, Harvard Medical School, Boston, MA, USA
3. Department of Psychiatry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA
4. Department of Clinical Sciences, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA

Correspondence: Dr R Kessler, Department of Health Care Policy, Harvard Medical School, 180 Longwood Ave, Boston, MA 2115, USA. E-mail: kessler@hcp.med.harvard.edu

Received 24 June 2008; Revised 27 January 2009; Accepted 28 January 2009; Published online 10 March 2009.

Abstract

Irritability is a diagnostic symptom of major depressive disorder (MDD) in children and adolescents but not in adults in both the Diagnostic and Statistical Manual of Mental Disorders, fourth edition (DSM-IV) and International Classification of Diseases (ICD-10) systems. We explore the importance of irritability for subtyping adult DSM-IV MDD in the National Comorbidity Survey Replication (NCS-R), a national US adult household survey. The WHO Composite International Diagnostic Interview (CIDI) was used to assess prevalence of many DSM-IV disorders in the lifetime and in the year before interview (12-month prevalence). MDD was assessed conventionally (that is, requiring either persistent sadness or loss of interest), but with irritability included as one of the Criterion A symptoms. We also considered the possibility that irritability might be a diagnostic symptom of adult MDD (that is, detect cases who had neither sad mood nor loss of interest). Twelve-month MDD symptom severity was assessed with the Quick Inventory of Depressive Symptomatology and role impairment with the Sheehan Disability Scale. After excluding bipolar spectrum disorders, irritability during depressive episodes was reported by roughly half of respondents with lifetime DSM-IV MDD. Irritability in the absence of either sad mood or loss of interest, in comparison, was rare. Irritability in MDD was associated with early age of onset, lifetime persistence, comorbidity with anxiety and impulse-control disorders, fatigue and self-reproach during episodes, and disability. Irritability was especially common in MDD among respondents in the age range 18–44 and students. Further investigation is warranted of distinct family aggregation, risk factors and treatment response. Consideration should also be given to including irritability as a nondiagnostic symptom of adult MDD in DSM-V and ICD-11.